Structure and chemical organization of the accessory olfactory bulb in the goat

Anat Rec (Hoboken). 2007 Mar;290(3):301-10. doi: 10.1002/ar.20505.


The structure and chemical composition of the accessory olfactory bulb (AOB) were examined in male and female goats. Sections were subjected to either Nissl staining, Klüver-Barrera staining, lectin histochemistry, or immunohistochemistry for nitric oxide synthase (NOS), neuropeptide Y (NPY), tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), and glutamic acid decarboxylase (GAD). The goat AOB was divided into four layers: the vomeronasal nerve layer (VNL), glomerular layer (GL), mitral/tufted (M/T) cell layer (MTL), and granule cell layer (GRL). Quantitative and morphometric analyses indicated that a single AOB contained 5,000-8,000 putative M/T cells with no sex differences, whereas the AOB was slightly larger in males. Of the 21 lectins examined, 7 specifically bound to the VNL and GL, and 1 bound not only to the VNL, but also to the MTL and GRL. In either of these cases, no heterogeneity of lectin staining was observed in the rostrocaudal direction. NOS-, TH-, DBH-, and GAD-immunoreactivity (ir) were observed in the MTL and GRL, whereas NPY-ir was present only in the GRL. In the GL, periglomerular cells with GAD-ir were found in abundance, and a subset of periglomerular cells containing TH-ir was also found. Double-labeling immunohistochemistry revealed that virtually all periglomerular cells containing TH-ir were colocalized with GAD-ir.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Communication
  • Animals
  • Dopamine beta-Hydroxylase / analysis
  • Female
  • Glutamate Decarboxylase / analysis
  • Goats / anatomy & histology*
  • Goats / metabolism
  • Immunohistochemistry
  • Lectins / analysis
  • Male
  • Neuropeptide Y / analysis
  • Nitric Oxide Synthase / analysis
  • Olfactory Bulb / chemistry*
  • Olfactory Bulb / cytology*
  • Olfactory Bulb / enzymology
  • Pheromones / metabolism
  • Staining and Labeling
  • Tyrosine 3-Monooxygenase / analysis


  • Lectins
  • Neuropeptide Y
  • Pheromones
  • Nitric Oxide Synthase
  • Tyrosine 3-Monooxygenase
  • Dopamine beta-Hydroxylase
  • Glutamate Decarboxylase