L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells

Nat Immunol. 2007 Jul;8(7):743-52. doi: 10.1038/ni1469. Epub 2007 May 27.


T lymphocytes lacking the lymph node-homing receptors L-selectin and CCR7 do not migrate to lymph nodes in the steady state. Instead, we found here that lymph nodes draining sites of mature dendritic cells or adjuvant inoculation recruited L-selectin-negative CCR7- effector and memory CD8+ T cells. This recruitment required CXCR3 expression on T cells and occurred through high endothelial venules in concert with lumenal expression of the CXCR3 ligand CXCL9. In reactive lymph nodes, recruited T cells established stable interactions with and killed antigen-bearing dendritic cells, limiting the ability of these dendritic cells to activate naive CD4+ and CD8+ T cells. The inducible recruitment of blood-borne effector and memory T cells to lymph nodes may represent a mechanism for terminating primary and limiting secondary immune responses.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Movement / immunology*
  • Cells, Cultured
  • Cytotoxicity, Immunologic*
  • Dendritic Cells / immunology*
  • Dendritic Cells / pathology
  • Immunologic Memory*
  • L-Selectin / biosynthesis
  • L-Selectin / metabolism*
  • Lymph Nodes / immunology*
  • Lymph Nodes / pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Molecular Sequence Data
  • Receptors, CCR7
  • Receptors, Chemokine / biosynthesis
  • Receptors, Chemokine / metabolism*
  • T-Lymphocyte Subsets / immunology


  • Ccr7 protein, mouse
  • Receptors, CCR7
  • Receptors, Chemokine
  • L-Selectin