Expression of HSP70 and JNK-related Proteins in Human Liver Cancer: Potential Effects on Clinical Outcome

Dig Liver Dis. 2007 Jul;39(7):663-70. doi: 10.1016/j.dld.2007.03.011. Epub 2007 May 24.

Abstract

Background: Activation of stress-activated protein kinase/c-Jun N-terminal kinase was inhibited in cells, in which heat shock protein70 was induced to a high level, indicating that heat shock protein70 might be anti-apoptosis protein.

Aim: We examined the expression of heat shock protein70 and c-Jun N-terminal kinase signal transduction pathway in human liver carcinoma to explore their relationship and clinical parameters.

Patients and methods: The expression of heat shock protein70, c-Jun N-terminal kinase1, c-Jun N-terminal kinase2 and c-Jun were detected immunohistochemically in 62 samples of liver cancer. Western blot was used to confirm immunostaining results.

Results: Heat shock protein70 expression showed a positive correlation with the malignant differentiation in liver carcinoma (r=0.449, P<0.0005). The expression of c-Jun N-terminal kinase1, c-Jun N-terminal kinase2, and c-Jun showed a negative correlation with the malignant differentiation in liver carcinoma (r=-0.351, P=0.005; r=-0.303, P=0.017; r=-0.302, P=0.017). Heat shock protein70 expression was correlated with c-Jun N-terminal kinase1 (r=-0.385, P=0.002), c-Jun N-terminal kinase2 (r=-0.309, P=0.015) and c-Jun (r=-0.302, P=0.017). Expression of heat shock protein70, as well as c-Jun N-terminal kinase1, was correlated with recurrence-free survival after the resection. Heat shock protein70 was associated with prognosis (P=0.004).

Conclusion: Expression of heat shock protein70 and c-Jun N-terminal kinase-related proteins might be an indicator of malignant potential in liver carcinoma. The balance between heat shock protein70 and c-Jun N-terminal kinase-related protein may increase the stability of liver cancer cells in stress. Negative expression of heat shock protein70 might be a protective factor of recurrence of liver carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blotting, Western
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / surgery
  • Female
  • HSP70 Heat-Shock Proteins / biosynthesis*
  • Humans
  • Immunohistochemistry
  • JNK Mitogen-Activated Protein Kinases / biosynthesis*
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / surgery
  • Male
  • Middle Aged
  • Mitogen-Activated Protein Kinase 8 / biosynthesis
  • Mitogen-Activated Protein Kinase 9 / biosynthesis
  • Neoplasm Proteins / biosynthesis
  • Signal Transduction
  • Treatment Outcome

Substances

  • HSP70 Heat-Shock Proteins
  • Neoplasm Proteins
  • Mitogen-Activated Protein Kinase 9
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 8