Expression stability of two housekeeping genes (18S rRNA and G3PDH) during in vitro maturation of follicular oocytes in buffalo (Bubalus bubalis)

Anim Reprod Sci. 2008 Jan 15;103(1-2):164-71. doi: 10.1016/j.anireprosci.2007.04.012. Epub 2007 Apr 22.


The present study was undertaken to evaluate the expression stability of two housekeeping genes (HKGs), 18S rRNA and G3PDH during in vitro maturation (IVM) of oocytes in buffalo, which qualifies their use as internal controls for valid qRT-PCR estimation of other oocyte transcripts. A semi quantitative RT-PCR system was used with optimised qRT-PCR parameters at exponential PCR cycle for evaluation of temporal expression pattern of these genes over 24 h of IVM. 18S rRNA was found more stable in its expression pattern than G3PDH.

MeSH terms

  • Animals
  • Buffaloes / genetics
  • Buffaloes / metabolism
  • Buffaloes / physiology*
  • Female
  • Gene Expression
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / biosynthesis*
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / genetics
  • Oocytes / enzymology
  • Oocytes / physiology*
  • Ovarian Follicle / enzymology
  • Ovarian Follicle / physiology*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Ribosomal, 18S / biosynthesis*
  • RNA, Ribosomal, 18S / genetics
  • Regression Analysis
  • Reverse Transcriptase Polymerase Chain Reaction / methods


  • RNA, Messenger
  • RNA, Ribosomal, 18S
  • Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)