Variation in fasting and non-fasting serum enterolactone concentrations in women of the Malmö Diet and Cancer cohort

Eur J Clin Nutr. 2008 Aug;62(8):1005-9. doi: 10.1038/sj.ejcn.1602811. Epub 2007 May 30.


Objectives: The aim of this study was to examine the variation of enterolactone from fasting and non-fasting blood of middle-aged healthy women eating a normal diet to determine the usefulness of a single sample in epidemiological studies.

Subjects and methods: Twenty-six women born between 1940 and 1950 were recruited within the Malmö Diet and Cancer cohort. Three non-fasting and two overnight fasting samples were collected from each individual during a 5-week period. Twenty-one participated in all measurements. Enterolactone concentrations were analyzed by time-resolved fluoroimmunoassay.

Results: The within-subject and between-subject variations (coefficient of variations, CV) were estimated to 59 and 89% respectively for fasting samples and 71 and 67% for non-fasting samples. The intraclass correlation coefficients (ICC) were estimated to 0.66 (95% confidence interval (CI) 0.35-0.84) for fasting and 0.48 (95% CI, 0.22-0.72) for non-fasting samples.

Conclusions: Although the estimated ICC for blood samples was moderate, it indicates that enterolactone levels of both fasting and non-fasting blood samples should be useful in future projects within the Malmö Diet and Cancer cohort.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / analysis
  • 4-Butyrolactone / blood
  • Biomarkers / blood
  • Cohort Studies
  • Diet
  • Fasting / blood*
  • Female
  • Fluoroimmunoassay / methods
  • Humans
  • Lignans / administration & dosage*
  • Lignans / analysis
  • Lignans / blood*
  • Lignans / metabolism
  • Middle Aged
  • Phytoestrogens / analysis
  • Phytoestrogens / blood*
  • Postprandial Period / physiology*
  • Prospective Studies
  • Risk Assessment
  • Risk Factors


  • Biomarkers
  • Lignans
  • Phytoestrogens
  • 4-Butyrolactone
  • 2,3-bis(3'-hydroxybenzyl)butyrolactone