Micro-bioreactor array for controlling cellular microenvironments

Lab Chip. 2007 Jun;7(6):710-9. doi: 10.1039/b700063d. Epub 2007 May 15.


High throughput experiments can be used to spatially and temporally investigate the many factors that regulate cell differentiation. We have developed a micro-bioreactor array (MBA) that is fabricated using soft lithography and contains twelve independent micro-bioreactors perfused with culture medium. The MBA enables cultivation of cells that are either attached to substrates or encapsulated in hydrogels, at variable levels of hydrodynamic shear, and with automated image analysis of the expression of cell differentiation markers. The flow and mass transport in the MBA were characterized by computational fluid dynamic (CFD) modeling. The representative MBA configurations were validated using the C2C12 cell line, primary rat cardiac myocytes and human embryonic stem cells (hESCs) (lines H09 and H13). To illustrate the utility of the MBA for controlled studies of hESCs, we established correlations between the expression of smooth muscle actin and cell density for three different flow configurations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Bioreactors*
  • Cell Count
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Computer Simulation
  • Culture Media
  • Embryonic Stem Cells / metabolism*
  • Humans
  • Models, Biological
  • Muscle, Smooth / metabolism
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / metabolism*
  • Nanotechnology*
  • Rats
  • Rheology
  • Shear Strength
  • Tissue Engineering / instrumentation
  • Tissue Engineering / methods*


  • Actins
  • Culture Media