The additional effect of a dentifrice on the instant efficacy of toothbrushing: a crossover study

J Periodontol. 2007 Jun;78(6):1011-6. doi: 10.1902/jop.2007.060339.


Background: Inconclusive evidence exists in the literature with regard to the additional (beneficial) mechanical effect of a dentifrice on plaque removal. A previous split-mouth study found that a dentifrice did not contribute to plaque removal. Because of limitations of the split-mouth model, a crossover design was used to evaluate whether a commercially available dentifrice had an additional effect on mechanical plaque removal during manual toothbrushing.

Methods: Thirty-six subjects were given a manual toothbrush and a standard dentifrice. After a 48-hour plaque accumulation, subjects brushed under supervision with or without a dentifrice (total time of 2 minutes) in a 2 x 2 crossover design.

Results: Plaque reductions were 50% with and 56% without the use of dentifrice. This 6% difference was statistically significant (P = 0.034). Explorative analysis showed that brushing without a dentifrice was more effective in removing plaque on the approximal surfaces.

Conclusions: The use of a dentifrice did not contribute to mechanical plaque removal during manual toothbrushing. It seemed that the mechanical action provided by the toothbrush was the main factor in the plaque-removing process.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Cariostatic Agents / therapeutic use
  • Cross-Over Studies
  • Dental Plaque / therapy*
  • Dentifrices / chemistry
  • Dentifrices / therapeutic use*
  • Female
  • Humans
  • Male
  • Reproducibility of Results
  • Sodium Fluoride / therapeutic use
  • Toothbrushing / methods*


  • Cariostatic Agents
  • Dentifrices
  • Sodium Fluoride