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, 26 (5), 745-52

Transactivation of miR-34a by p53 Broadly Influences Gene Expression and Promotes Apoptosis

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Transactivation of miR-34a by p53 Broadly Influences Gene Expression and Promotes Apoptosis

Tsung-Cheng Chang et al. Mol Cell.

Abstract

The p53 tumor suppressor protein is a critical regulator of the cellular response to cancer-initiating insults such as genotoxic stress. In this report, we demonstrate that microRNAs (miRNAs) are important components of the p53 transcriptional network. Global miRNA expression analyses identified a cohort of miRNAs that exhibit p53-dependent upregulation following DNA damage. One such miRNA, miR-34a, is commonly deleted in human cancers and, as shown here, frequently absent in pancreatic cancer cells. Characterization of the miR-34a primary transcript and promoter demonstrates that this miRNA is directly transactivated by p53. Expression of miR-34a causes dramatic reprogramming of gene expression and promotes apoptosis. Much like the known set of p53-regulated genes, miR-34a-responsive genes are highly enriched for those that regulate cell-cycle progression, apoptosis, DNA repair, and angiogenesis. Therefore, it is likely that an important function of miR-34a is the modulation and fine-tuning of the gene expression program initiated by p53.

Figures

Figure 1
Figure 1. miR-34a is induced by p53 following DNA damage
(A) Northern blot analysis of miRNA expression in p53WT and p53-/- HCT116 cells with or without adriamycin treatment. Quantification of the miRNA signals under each condition, normalized to U6 snRNA expression, is shown on the right. (B) Phylogenetic conservation of the genomic region near the transcription start site of the miR-34a primary transcript. VISTA (http://genome.lbl.gov/vista/index.shtml) was used to generate pairwise alignments between human and mouse and human and rat. The graph is a plot of nucleotide identity for a 100 base-pair sliding window centered at a given position. Position +1 of pri-miR-34a is the most 5′ transcription start site mapped. The magnified sequence shows the location and evolutionary conservation of the p53 binding site (in gray box). Mutations introduced into the promoter reporter construct (P1mut in panel C) are shown in red. (C) Activity of promoter constructs in p53WT and p53-/-cells. The arrow above construct P1 indicates the position of the transcription start site. Filled circles show the position of the p53 binding site. Error bars represent standard deviations from three independent transfections each measured in triplicate. (D) Genotoxic stress activates the miR-34a promoter. The indicated promoter constructs were transfected into p53WT or p53-/- cells with or without subsequent exposure to adriamycin.
Figure 2
Figure 2. Expression of miR-34a promotes apoptosis
(A) p53WT and p53-/- cells were transfected with synthetic miR-34a or a control oligonucleotide and DNA content was monitored by flow cytometry. The fraction of apoptotic cells (sub-G1) is indicated. (B) Cell death was monitored by Annexin V staining and flow cytometry. The right lower quandrant of each plot contains early apoptotic cells whereas the right upper quadrant contains late apoptotic cells. This experiment was repeated three independent times and similar results were obtained each time. Panels (A) and (B) come from two separate experiments.
Figure 3
Figure 3. Expression of miR-34a is frequently lost in pancreatic cancer cells
Northern blot analysis of miR-34a expression in non-transformed pancreatic ductal epithelial cell lines (HPNE and HPDE, in bold) and in pancreatic cancer cell lines. Relative expression of miR-34a in each cell line, normalized to U6 snRNA expression, is shown below the blots. Dashes indicate undetectable miR-34a expression.
Figure 4
Figure 4. Expression of miR-34a leads to widespread alterations in gene expression
(A) Northern blot showing miR-34a expression in retrovirally-infected cell populations. (B) Northern blot validation of selected expression changes observed by microarray analysis. (C) Sites complementary to the miR-34a seed are enriched in the 3′ UTRs of downregulated transcripts. The graph shows the frequency of a site complementary to the miR-34a seed in the 3′ UTRs of the top 100 downregulated genes (2.364-fold or greater downregulation), the unchanged genes, and the genes upregulated by 2.364-fold or greater.

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