Automated mass immunization of poultry: the prospect for nonreplicating human adenovirus-vectored in ovo vaccines

Expert Rev Vaccines. 2007 Jun;6(3):457-65. doi: 10.1586/14760584.6.3.457.


Automated in ovo vaccination is an efficient method for mass immunization of poultry. Although in ovo vaccination has been used to mass immunize chickens against several infectious diseases, there are common poultry diseases for which in ovo-compatible vaccines are not commercially available. It was recently demonstrated that in ovo administration of a nonreplicating human adenovirus vector encoding an avian influenza virus hemagglutinin induced protective immunity against highly pathogenic avian influenza. The advantages of this new class of poultry vaccine include in ovo delivery of a wide variety of pathogen-derived antigens, high potency in a single-dose regimen, rapid production in response to increased demand, no replication of the vector, no pre-existing immunity to human adenovirus in chickens, compatibility with automated in ovo administration and no interference with epidemiological surveys of natural infections.

Publication types

  • Review

MeSH terms

  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / immunology*
  • Animals
  • Chickens
  • Communicable Disease Control / methods
  • Communicable Diseases / immunology
  • Communicable Diseases / veterinary*
  • Genetic Vectors*
  • Influenza in Birds / prevention & control*
  • Mass Vaccination / methods
  • Mass Vaccination / veterinary*
  • Poultry Diseases / prevention & control*
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Viral Vaccines / immunology*


  • Vaccines, Synthetic
  • Viral Vaccines