Imaging voltage-dependent cell motions with heterodyne Mach-Zehnder phase microscopy

Opt Lett. 2007 Jun 1;32(11):1572-4. doi: 10.1364/ol.32.001572.

Abstract

We describe a heterodyne Mach-Zehnder interferometric microscope capable of quantitative phase imaging of biological samples with subnanometer sensitivity and frame rates up to 10 kHz. We use the microscope to image cultured neurons and measure nanometer-scale voltage-dependent motions in cells expressing the membrane motor protein prestin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anion Transport Proteins / metabolism
  • Cell Line
  • Cell Movement
  • Electrochemistry / methods
  • Electrophysiology / methods
  • Equipment Design
  • Humans
  • Interferometry / methods*
  • Microscopy, Phase-Contrast / methods*
  • Models, Biological
  • Neurons / metabolism
  • Rats
  • Sensitivity and Specificity
  • Sulfate Transporters
  • Time Factors
  • Transfection

Substances

  • Anion Transport Proteins
  • SLC26A5 protein, human
  • Sulfate Transporters