Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions

Biosens Bioelectron. 2007 Sep 30;23(2):261-8. doi: 10.1016/j.bios.2007.04.009. Epub 2007 May 4.


A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6mm length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm2). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Blood Coagulation / physiology*
  • Blood Vessels / cytology
  • Blood Vessels / physiology*
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Flow Injection Analysis / instrumentation*
  • Flow Injection Analysis / methods
  • Humans
  • Platelet Adhesiveness / physiology*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Shear Strength
  • Surface Plasmon Resonance / instrumentation*
  • Surface Plasmon Resonance / methods