Tobacco smoke exposure is associated with attenuated endothelial function in 11-year-old healthy children

Circulation. 2007 Jun 26;115(25):3205-12. doi: 10.1161/CIRCULATIONAHA.106.674804. Epub 2007 Jun 4.


Background: Passive smoking is associated with early arterial damage in adults, but its effect on endothelial function in children is unknown.

Methods and results: Serum cotinine concentration was measured annually in children between 8 and 11 years of age who had participated since infancy in a randomized, prospective atherosclerosis prevention trial (Special Turku Coronary Risk Factor Intervention Project for children [STRIP]). At age 11, endothelium-dependent flow-mediated vasodilatory responses of the brachial artery were examined with high-resolution ultrasound in 402 children. These children were divided into 3 groups according to serum cotinine concentrations: the noncotinine group (nondetectable cotinine, n=229), the low cotinine group (cotinine between 0.2 and 1.6 ng/mL, n=134), and the top decile cotinine group (cotinine > or = 1.7 ng/mL, n=39). Longitudinal cotinine data in children aged 8 to 11 years and ultrasound studies were available in 327 children. At age 11, the increase in cotinine concentration was associated with attenuated peak flow-mediated dilation response (mean+/-SD: the noncotinine group 9.10+/-3.88%, the low-cotinine group 8.57+/-3.78%, and the top-decile cotinine group 7.73+/-3.85%; P=0.03 for trend). Similarly, total dilation response (the area under the dilation response versus time curve between 40 and 180 seconds after hyperemia) was affected by the cotinine level (P=0.02 for trend). These trends were not explained by traditional atherosclerosis risk factors. Arterial measures and passive smoking showed even stronger associations when longitudinal cotinine data were used (peak flow-mediated dilation, P=0.01 for trend; total dilation response, P=0.008 for trend).

Conclusions: Exposure to environmental tobacco smoke confirmed by serum cotinine concentrations impairs endothelial function in a dose-dependent manner in 11-year-old children.

Trial registration: NCT00223600.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Atherosclerosis / etiology
  • Atherosclerosis / prevention & control
  • Brachial Artery / diagnostic imaging
  • Brachial Artery / physiopathology*
  • Child
  • Cohort Studies
  • Cotinine / blood*
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / physiopathology*
  • Female
  • Finland / epidemiology
  • Follow-Up Studies
  • Hemorheology
  • Humans
  • Life Style
  • Male
  • Nitric Oxide / physiology
  • Observer Variation
  • Parents / psychology
  • Prospective Studies
  • Risk Factors
  • Surveys and Questionnaires
  • Tobacco Smoke Pollution / adverse effects*
  • Tobacco Smoke Pollution / prevention & control
  • Ultrasonography
  • Vasodilation


  • Tobacco Smoke Pollution
  • Nitric Oxide
  • Cotinine

Associated data