Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata

Med Vet Entomol. 2007 Jun;21(2):127-31. doi: 10.1111/j.1365-2915.2007.00668.x.

Abstract

Low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography-mass spectrometry analysis, three compounds were obtained: p-hydroxybenzoic acid (molecular weight 138 Da), p-hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro-dipyrrolo[1,2-a;1',2'-d] pyrazine-5,10-dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa, and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification*
  • Anti-Bacterial Agents / pharmacology
  • Chromatography, High Pressure Liquid / veterinary
  • Diptera / chemistry*
  • Hemolymph / chemistry
  • Hydroxybenzoates / chemistry
  • Hydroxybenzoates / isolation & purification*
  • Hydroxybenzoates / pharmacology
  • Larva / chemistry
  • Microbial Sensitivity Tests
  • Micrococcus luteus / drug effects
  • Molecular Weight
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / isolation & purification*
  • Peptides, Cyclic / pharmacology
  • Phenylacetates / chemistry
  • Phenylacetates / isolation & purification*
  • Phenylacetates / pharmacology
  • Pseudomonas aeruginosa / drug effects
  • Stem Cells
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Hydroxybenzoates
  • Peptides, Cyclic
  • Phenylacetates
  • cyclo(prolylprolyl)
  • 4-hydroxyphenylacetic acid