Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42

Mol Plant Microbe Interact. 2007 Jun;20(6):619-26. doi: 10.1094/MPMI-20-6-0619.

Abstract

Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (deltatrpBA) and E102 (deltatrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (deltaysnE, putative IAA transacetylase) and E105 (deltayhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Araceae / growth & development*
  • Araceae / metabolism
  • Araceae / microbiology*
  • Bacillus / classification*
  • Bacillus / genetics
  • Bacillus / physiology*
  • Biological Assay
  • Gas Chromatography-Mass Spectrometry
  • Genes, Bacterial
  • Indoleacetic Acids / analysis
  • Indoleacetic Acids / metabolism*
  • Mutation / genetics
  • Sequence Homology, Nucleic Acid
  • Tryptophan / biosynthesis
  • Tryptophan / metabolism*
  • Tryptophan / pharmacology

Substances

  • Indoleacetic Acids
  • indoleacetic acid
  • Tryptophan