How a plant lectin recognizes high mannose oligosaccharides

Plant Physiol. 2007 Aug;144(4):1733-41. doi: 10.1104/pp.107.100867. Epub 2007 Jun 7.


The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man-9. Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-. Isothermal titration calorimetry titration experiments using Man-5, Man-9, and the Man-9-containing glycoprotein soybean (Glycine max) agglutinin as ligands confirm the monovalence of Man-9 and show a 4-times higher affinity for Man-9 when it is presented to P. angolensis seed lectin in a glycoprotein context.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Crystallography, X-Ray
  • Ligands
  • Mannose / metabolism*
  • Mannose-Binding Lectins / chemistry
  • Mannose-Binding Lectins / metabolism*
  • Molecular Conformation
  • Oligosaccharides / metabolism*
  • Pterocarpus / metabolism*
  • Seeds / metabolism*
  • Thermodynamics


  • Ligands
  • Mannose-Binding Lectins
  • Oligosaccharides
  • Mannose

Associated data

  • PDB/2PHF
  • PDB/2PHR
  • PDB/2PHT
  • PDB/2PHU
  • PDB/2PHW
  • PDB/2PHX