Polony multiplex analysis of gene expression (PMAGE) in mouse hypertrophic cardiomyopathy

Science. 2007 Jun 8;316(5830):1481-4. doi: 10.1126/science.1137325.


We describe a sensitive mRNA profiling technology, PMAGE (for "polony multiplex analysis of gene expression"), which detects messenger RNAs (mRNAs) as rare as one transcript per three cells. PMAGE incorporates an improved ligation-based method to sequence 14-nucleotide tags derived from individual mRNA molecules. One sequence tag from each mRNA molecule is amplified onto a separate 1-micrometer bead, denoted as a polymerase colony or polony, and about 5 million polonies are arrayed in a flow cell for parallel sequencing. Using PMAGE, we identified early transcriptional changes that preceded pathological manifestations of hypertrophic cardiomyopathy in mice carrying a disease-causing mutation. PMAGE provided a comprehensive profile of cardiac mRNAs, including low-abundance mRNAs encoding signaling molecules and transcription factors that are likely to participate in disease pathogenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiomyopathy, Hypertrophic / genetics*
  • Cardiomyopathy, Hypertrophic / pathology
  • Cardiomyopathy, Hypertrophic / physiopathology
  • DNA, Complementary
  • Fibrosis / genetics
  • Fibrosis / pathology
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation*
  • Gene Library
  • Heart Ventricles / metabolism
  • Mice
  • Mutation
  • Myocardial Contraction
  • Myocardium / metabolism*
  • Myosin Heavy Chains / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Templates, Genetic
  • Transcription Factors / genetics
  • Transcription, Genetic*
  • Ventricular Myosins / genetics


  • DNA, Complementary
  • RNA, Messenger
  • Transcription Factors
  • Ventricular Myosins
  • Myosin Heavy Chains