Abstract
The folA gene was identified as a new member of the TyrR regulon by genomic SELEX. Binding of TyrR to two sites in folA activated its transcription. Mutations in the N-terminal or central domain of TyrR, the alpha subunit of RNA polymerase, or integration host factor all abolished activation of the folA promoter.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Escherichia coli / genetics*
-
Escherichia coli Proteins / chemistry*
-
Escherichia coli Proteins / isolation & purification
-
Gene Expression Regulation, Bacterial
-
Regulon / genetics*
-
Repressor Proteins / chemistry*
-
Repressor Proteins / genetics
-
Repressor Proteins / physiology
-
Tetrahydrofolate Dehydrogenase / metabolism
Substances
-
Escherichia coli Proteins
-
Repressor Proteins
-
TyrR protein, E coli
-
Tetrahydrofolate Dehydrogenase