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. 2007 Aug;42(4):321-4.
doi: 10.1016/j.ymeth.2007.01.006.

Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

Satyajit D Sarker  1 , Lutfun Nahar, Yashodharan Kumarasamy
Affiliations
Free PMC article

Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

Satyajit D Sarker et al. Methods. 2007 Aug.
Free PMC article

Abstract

The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.

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Figures

Fig. 1
Fig. 1
Plates after 24 h in modified resazurin assay [pink colour indicates growth and blue means inhibition of growth; the test organism was Staphylococcus aureus; C1, sterility control (test compound in serial dilution + broth + saline + indicator), no bacteria; C2, control without drug (bacteria + broth + indicator); C3, positive control (ciprofloxacin in serial dilution + broth + indicator + bacteria); A–D, Test compound/extract (in serial dilution in wells 1–12 + broth + indicator + bacteria)].
Fig. 2
Fig. 2
Structures of phytochemicals tested in the modified resazurin assay.
See this image and copyright information in PMC

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References

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