Drosophila dSet2 functions in H3-K36 methylation and is required for development

Biochem Biophys Res Commun. 2007 Aug 3;359(3):784-9. doi: 10.1016/j.bbrc.2007.05.189. Epub 2007 Jun 4.


Lysine methylation has important functions in biological processes that range from heterochromatin formation to transcription regulation. Here, we demonstrate that Drosophila dSet2 encodes a developmentally essential histone H3 lysine 36 (K36) methyltransferase. Larvae subjected to RNA interference-mediated (RNAi) suppression of dSet2 lack dSet2 expression and H3-K36 methylation, indicating that dSet2 is the sole enzyme responsible for this modification in Drosophila melanogaster. dSet2 RNAi blocks puparium formation and adult development, and causes partial (blister) separation of the dorsal and ventral wing epithelia, defects suggesting a failure of the ecdysone-controlled genetic program. A transheterozygous EcR null mutation/dSet2 RNAi combination produces a complete (balloon) separation of the wing surfaces, revealing a genetic interaction between EcR and dSet2. Using immunoprecipitation, we demonstrate that dSet2 associates with the hyperphosphorylated form of RNA polymerase II (RNAPII).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development*
  • Drosophila melanogaster / metabolism*
  • Gene Expression Regulation, Developmental*
  • Histone-Lysine N-Methyltransferase / genetics
  • Histone-Lysine N-Methyltransferase / metabolism*
  • Histones / metabolism*
  • Lysine / genetics
  • Lysine / metabolism*
  • Methylation
  • Phosphorylation
  • Protein Binding
  • RNA Polymerase II / metabolism
  • Receptors, Steroid / genetics
  • Receptors, Steroid / metabolism


  • Drosophila Proteins
  • Histones
  • Receptors, Steroid
  • ecdysone receptor
  • Histone-Lysine N-Methyltransferase
  • Set2 protein, Drosophila
  • RNA Polymerase II
  • Lysine