Abstract
Genes whose expression patterns are altered in a cell line immortalized by mutant p53 were isolated by differential screening of a cDNA library. Levels of alpha 1 (I) collagen mRNA were reduced in the majority of immortalized cell lines which greatly overproduced the transfected mutant p53. This may reflect a co-selection during the establishment of the cell lines, rather than a direct effect of p53 on alpha 1 (I) collagen gene expression. On the other hand, a more direct relationship could be demonstrated between the expression of activated ras and a reduction in alpha 1 (I) collagen mRNA. Such reduction could partially account for the effects of ras on cell shape and cell proliferation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Blotting, Northern
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Cell Transformation, Neoplastic / genetics
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Collagen / genetics*
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Down-Regulation / genetics
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Electrophoresis, Gel, Two-Dimensional
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Genes, p53*
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Genes, ras*
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Molecular Sequence Data
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Mutation / genetics
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Protein Precursors / genetics
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Proto-Oncogene Proteins p21(ras) / genetics
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Rats
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Rats, Inbred F344
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Sequence Alignment
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Tumor Suppressor Protein p53 / genetics
Substances
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Protein Precursors
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Tumor Suppressor Protein p53
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Collagen
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Proto-Oncogene Proteins p21(ras)
Associated data
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GENBANK/S55807
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GENBANK/S55811
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GENBANK/S55812
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GENBANK/S55814
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GENBANK/S55817
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GENBANK/S73266
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GENBANK/S73302
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GENBANK/S73643
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GENBANK/X64359
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GENBANK/X64360