Cloning, sequencing, and mapping of an alpha-actinin gene from the nematode Caenorhabditis elegans

Cell Motil Cytoskeleton. 1991;20(1):69-78. doi: 10.1002/cm.970200108.


The dense-bodies in the body wall muscle of the nematode Caenorhabditis elegans function to anchor the actin thin filaments to the adjacent sarcolemma. One of the major components of the dense-bodies is the actin-binding protein alpha-actinin. To facilitate a genetic analysis of alpha-actinin, we have cloned a cDNA encoding the nematode protein, identified its position on the nematode physical map, and developed a unique PCR based approach to test the position of the cloned gene relative to known genetic deletions. The peptide sequence deduced from the cDNA shows that, apart from a few exceptional regions, the nematode protein shows strong similarity to other known alpha-actinins. Its position on the genetic map shows that none of the known muscle affecting mutations identified in C. elegans are in this alpha-actinin gene. This gene has been given the name atn-1 (alpha-actinin-1).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actinin / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis / genetics*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Mutational Analysis
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Sequence Homology, Nucleic Acid


  • Actinin