Miniaturized asymmetrical flow field-flow fractionation: application to biological vesicles

J Sep Sci. 2007 May;30(7):1082-7. doi: 10.1002/jssc.200600394.


Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fractionation, Field Flow / instrumentation*
  • Fractionation, Field Flow / methods*
  • Miniaturization / instrumentation*
  • Particle Size
  • Polystyrenes / chemistry
  • Proteins / chemistry
  • Surface Properties
  • Time Factors


  • Polystyrenes
  • Proteins