Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?

EMBO J. 1991 Dec;10(13):4197-207. doi: 10.1002/j.1460-2075.1991.tb04998.x.


We have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally similar sequence downstream of the human alpha 2 globin gene reveals that both signals function in an orientation dependent manner, with subfragments of the whole signal displaying partial effects. In the case of the C2 termination sequence a protein binds within it, and is partially responsible for the termination effect. We further demonstrate that the same (or closely related) protein binds to the ME1a1 site in the murine c-myc promoter, which has been implicated in c-myc attenuation. We suggest that the termination/pause sequences positioned downstream of a gene's poly(A) site may constitute the general signals that elicit transcriptional termination in genes transcribed by RNA polymerase II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Complement C2 / genetics*
  • Complement Factor B / genetics*
  • DNA Fingerprinting
  • Globins / genetics
  • Globins / metabolism
  • Humans
  • Liver / enzymology
  • Liver / metabolism
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins c-myc / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Terminator Regions, Genetic*
  • Transfection
  • beta-Galactosidase / metabolism


  • Complement C2
  • Proto-Oncogene Proteins c-myc
  • Globins
  • Chloramphenicol O-Acetyltransferase
  • beta-Galactosidase
  • Complement Factor B