Proteomics analyses of ovarian cancer using genetically defined human ovarian cancer models

Front Biosci. 2007 Sep 1:12:5166-74. doi: 10.2741/2556.

Abstract

Using a genetically defined human ovarian cancer model, we have analyzed the protein expression profile of human ovarian cancer cells established by oncogenic RAS transformation. Cells were immortalized by retroviral transfection of SV40 t/T antigens and the catalytic subunit of telomerase (hTERT). Careful analyses of protein targets associated with oncogenic transformation have enabled us to identify several novel signaling pathways that play important roles in oncogenic transformation of human ovarian epithelial cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / physiology
  • Cell Transformation, Neoplastic / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Endosomal Sorting Complexes Required for Transport
  • Female
  • Humans
  • Models, Biological
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / metabolism*
  • Proteomics*
  • Signal Transduction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured
  • Up-Regulation
  • ras Proteins / metabolism

Substances

  • DNA-Binding Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Transcription Factors
  • Tsg101 protein
  • ras Proteins