Anxiolytic-like effects of sinapic acid in mice

Life Sci. 2007 Jun 27;81(3):234-40. doi: 10.1016/j.lfs.2007.05.007. Epub 2007 May 21.


Sinapic acid is a phenylpropanoid compound and is found in various herbal materials and high-bran cereals. With the exception of its antioxidant activities, the pharmacological properties of sinapic acid have been rarely reported. The purpose of this study was to characterize the putative anxiolytic-like properties of sinapic acid using an elevated plus-maze (EPM) and hole-board test. Control mice were orally treated with an equal volume of vehicle (10% Tween 80 solution), and positive control mice were treated with diazepam (1 mg/kg, i.p.). Sinapic acid (4 mg/kg, p.o.) significantly increased the percentages of time spent in the open arms of the EPM test (P<0.05). In the hole-board test, sinapic acid also significantly increased the number of head-dips at 4 mg/kg (P<0.05). In addition, the anxiolytic-like properties of sinapic acid examined in the EPM test were blocked by flumazenil or bicuculline, which are GABA(A) antagonists. Moreover, sinapic acid markedly potentiated GABA current in single cortical neurons in a dose-dependant manner, and reactive I(GABA) increased to 1.8 times at 1 muM of sinapic acid. These results suggested that sinapic acid is a prominent anxiolytic agent, and that its anxiolytic-like effects are mediated via GABA(A) receptors and potentiating Cl(-) currents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Anxiety Agents*
  • Anxiety / drug therapy
  • Anxiety / psychology
  • Bicuculline / pharmacology
  • Cerebral Cortex / cytology
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / physiology
  • Chloride Channels / drug effects
  • Coumaric Acids / pharmacology*
  • Diazepam / pharmacology
  • Electrophysiology
  • Flumazenil / pharmacology
  • GABA Antagonists / pharmacology
  • GABA Modulators / pharmacology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Motor Activity / drug effects
  • Muscle Relaxation / drug effects
  • Muscle, Smooth / drug effects
  • Neurons / drug effects
  • Neurotransmitter Agents / physiology
  • Piperazines / pharmacology
  • Postural Balance / drug effects
  • Pyridines / pharmacology
  • Serotonin Antagonists / pharmacology
  • gamma-Aminobutyric Acid / pharmacology


  • Anti-Anxiety Agents
  • Chloride Channels
  • Coumaric Acids
  • GABA Antagonists
  • GABA Modulators
  • Neurotransmitter Agents
  • Piperazines
  • Pyridines
  • Serotonin Antagonists
  • Flumazenil
  • gamma-Aminobutyric Acid
  • sinapinic acid
  • N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide
  • Diazepam
  • Bicuculline