Active compounds from Actinidia deliciosa were separated and identified. Column chromatography was used for isolation. Physicochemical and spectroscopic analysis were employed for structural identification. 5 compounds were isolated and identified as 2alpha, 3beta, 19, 23-tetrahydroxylolean-12-en-28-oic acid( I ), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid( II ) , 2alpha, 3beta, 19,23-tetra-hydroxyl-urs-12-en-oic acid( I ) , undecanoic acid( IV) ,beta-sitoserol( V ). I , IV and V were isolated from roots of Actinidia deliciosa for the first time.