Validation of the MycoAlign System for Mycobacterium Spp. Identification

Diagn Microbiol Infect Dis. 2007 Sep;59(1):105-8. doi: 10.1016/j.diagmicrobio.2007.04.012. Epub 2007 Jun 15.

Abstract

Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

Publication types

  • Validation Study

MeSH terms

  • Bacterial Proteins / genetics*
  • Chaperonin 60
  • Chaperonins / genetics*
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Mycobacterium / classification*
  • Mycobacterium / genetics
  • RNA, Ribosomal, 16S / genetics*
  • RNA, Ribosomal, 23S / genetics*
  • Reagent Kits, Diagnostic

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S
  • Reagent Kits, Diagnostic
  • heat-shock protein 65, Mycobacterium
  • Chaperonins