The LS electromagnetic band (1-4 GHz) is widely used both in domestic and industrial domains. Several studies suggested that the biological systems would exhibit a specific sensitivity to the 2.45 GHz microwaves (water resonance frequency). Potential human health hazards and especially a disruption of the cholinergic system have been reported, due to exposure to microwaves even at low power density. This work presents a multiparametric study of freely moving rat where neurophysiology was investigated during 70 hours using neurochemical (microdialysis technique), electrophysiological, behavioral (vigilance stages quantification) and thermophysiological approaches. The rats were exposed 24 hours to a 2.45 GHz pulsed electromagnetic field at low power density. In this exposure conditions, no significant effect have been reported.