Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors

Nat Methods. 2007 Jul;4(7):551-3. doi: 10.1038/nmeth1059. Epub 2007 Jun 17.


Zebrafish are a valuable vertebrate model to study carcinogenesis, but noninvasive imaging is challenging because adult fish are not transparent. Here we show that tumors could be readily detected in vivo using high-resolution microscopic ultrasound in zebrafish. We successfully obtained tissue perfusion calculations and cellular aspirates, and analyzed tumor progression and response to treatment. Ultrasound biomicroscopy allows longitudinal studies of tumor development and real-time assessment of therapeutic effects in zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Liver Neoplasms, Experimental / diagnostic imaging*
  • Liver Neoplasms, Experimental / pathology
  • Microscopy, Acoustic / methods*
  • Zebrafish*