CYSTUS052, a polyphenol-rich plant extract, exerts anti-influenza virus activity in mice

Antiviral Res. 2007 Oct;76(1):1-10. doi: 10.1016/j.antiviral.2007.04.001. Epub 2007 May 15.

Abstract

Influenza, a respiratory disease caused by influenza viruses, is still a worldwide threat with a high potential to cause a pandemic. Beside vaccination, only two classes of drugs are available for antiviral treatment against the pathogen. Here we show that CYSTUS052, a plant extract from a special variety of Cistus incanus that is rich in polymeric polyphenols, exhibits antiviral activity against a highly pathogenic avian influenza A virus (H7N7) in cell culture and in a mouse infection model. In vitro and in vivo treatment was performed with an aerosol formulation, because the bioavailability of high molecular weight polyphenols is poor. In MDCK cells, a 90% reduction of plaque numbers on cells pre-incubated with the plant extract was achieved. For in vivo experiments we used a novel monitoring system for influenza A virus-infected mice that allows measurement of body temperature and gross motor-activity of the animals. Mice treated with CYSTUS052 did not develop disease, showed neither differences in their body temperature nor differences in their gross motor-activity and exhibited no histological alterations of the bronchiolus epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerosols
  • Animals
  • Antiviral Agents / administration & dosage
  • Antiviral Agents / chemistry*
  • Bronchi / pathology
  • Citrus*
  • Dogs
  • Drug Administration Schedule
  • Drug Evaluation, Preclinical
  • Epithelial Cells / pathology
  • Female
  • Fever / pathology
  • Flavonoids*
  • Influenza A Virus, H7N7 Subtype* / growth & development
  • Inhalation
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Movement
  • Orthomyxoviridae Infections / drug therapy*
  • Orthomyxoviridae Infections / physiopathology
  • Phenols* / administration & dosage
  • Phenols* / chemistry
  • Plant Extracts / administration & dosage
  • Plant Extracts / chemistry*
  • Polyphenols
  • Viral Plaque Assay

Substances

  • Aerosols
  • Antiviral Agents
  • CYSTUS052
  • Flavonoids
  • Phenols
  • Plant Extracts
  • Polyphenols