Activation of FOXO3a by the green tea polyphenol epigallocatechin-3-gallate induces estrogen receptor alpha expression reversing invasive phenotype of breast cancer cells

Cancer Res. 2007 Jun 15;67(12):5763-70. doi: 10.1158/0008-5472.CAN-06-4327.

Abstract

Previously, we showed that the bioactive green tea polyphenol epigallocatechin-3-gallate (EGCG) inhibits growth in soft agar of breast cancer cells with Her-2/neu overexpression. Using gene expression profiling, here we show that EGCG treatment of Her-2/neu-driven mammary tumor cells alters the expression of key regulators in the epithelial to mesenchymal transition (EMT) pathway, reducing invasive phenotype. Specifically, the epithelial genes E-cadherin, gamma-catenin, MTA3, and estrogen receptor alpha (ERalpha) were up-regulated by EGCG, whereas the proinvasive snail gene was down-regulated. Consistently, EGCG inhibited branching colony growth and invasion in Matrigel. EGCG treatment similarly inhibited invasive phenotype of mouse mammary tumor cells driven by Nuclear Factor-kappaB c-Rel and protein kinase CK2, frequently found overexpressed in human breast disease. Recently, we identified the Forkhead box O transcription factor FOXO3a as a major transcriptional regulator of ERalpha. Given the pivotal role of ERalpha in preventing EMT, we hypothesized that the activation of FOXO3a by EGCG plays an important role in the observed reversal of invasive phenotype in ERalpha-positive breast cancer cells. EGCG treatment activated FOXO3a. Ectopic expression of a constitutively active FOXO3a overrode transforming growth factor-beta1-mediated invasive phenotype and induced a more epithelial phenotype, which was dependent on ERalpha expression and signaling. Conversely, a dominant negative FOXO3a reduced epithelial phenotype of ERalpha-low breast cancer cells. These results identify, for the first time, a role for FOXO3a in the inhibition of invasive phenotype in breast cancer cells with active ERalpha signaling and elucidate a novel mechanism whereby EGCG represses EMT of breast cancer cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticarcinogenic Agents / pharmacology*
  • Beverages
  • Breast Neoplasms / pathology*
  • Cadherins / drug effects
  • Cadherins / genetics
  • Cadherins / metabolism
  • Catechin / analogs & derivatives*
  • Catechin / pharmacology
  • Estrogen Receptor alpha / drug effects*
  • Estrogen Receptor alpha / genetics
  • Estrogen Receptor alpha / metabolism
  • Female
  • Flavonoids / pharmacology
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors / drug effects*
  • Forkhead Transcription Factors / metabolism
  • Gene Expression / drug effects*
  • Gene Expression Profiling
  • Humans
  • Immunoblotting
  • Neoplasm Invasiveness / genetics
  • Neoplasm Proteins / drug effects
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Phenols / pharmacology
  • Phenotype
  • Polyphenols
  • Receptor, ErbB-2 / drug effects
  • Receptor, ErbB-2 / genetics
  • Receptor, ErbB-2 / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Snail Family Transcription Factors
  • Transcription Factors / drug effects
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transfection
  • gamma Catenin / drug effects
  • gamma Catenin / genetics
  • gamma Catenin / metabolism

Substances

  • Anticarcinogenic Agents
  • Cadherins
  • Estrogen Receptor alpha
  • FOXO3 protein, human
  • Flavonoids
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • MTA3 protein, human
  • Neoplasm Proteins
  • Phenols
  • Polyphenols
  • Snail Family Transcription Factors
  • Transcription Factors
  • gamma Catenin
  • Catechin
  • epigallocatechin gallate
  • Receptor, ErbB-2