Oxidation-reduction states of NADH in vivo: from animals to clinical use

Mitochondrion. 2007 Sep;7(5):330-9. doi: 10.1016/j.mito.2007.05.001. Epub 2007 May 10.

Abstract

Mitochondrial dysfunction is part of many pathological states in patients, such as sepsis or stroke. Presently, the monitoring of mitochondrial function in patients is extremely rare, even though NADH redox state is routinely measured in experimental animals. In this article, we describe the scientific backgrounds and practical use of mitochondrial NADH fluorescence measurement that was applied to patients in the past few years. In addition to NADH, we optically measured the microcirculatory blood flow and volume, as well as HbO(2) oxygenation, from the same tissue area. The four detected parameters provide real time data on tissue viability, which is critical for patients monitoring.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Circulation / physiology
  • Gerbillinae
  • Humans
  • Male
  • Mitochondria / metabolism*
  • Mitochondrial Diseases / metabolism
  • Monitoring, Physiologic
  • NAD / analysis
  • NAD / metabolism*
  • Oxidation-Reduction
  • Spectrometry, Fluorescence

Substances

  • NAD