Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Nucleic Acids Res. 2007;35(13):4223-37. doi: 10.1093/nar/gkm389. Epub 2007 Jun 18.


The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply 'Lab on a chip' systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adsorption
  • DNA, Bacterial / analysis
  • DNA, Viral / analysis
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / trends*
  • Polymerase Chain Reaction / instrumentation*
  • Polymerase Chain Reaction / trends*
  • Temperature
  • Time Factors


  • DNA, Bacterial
  • DNA, Viral