A specific real-time PCR assay for the detection of Bordetella pertussis

J Med Microbiol. 2007 Jul;56(Pt 7):918-920. doi: 10.1099/jmm.0.46947-0.


A novel real-time PCR (RT-PCR) assay was developed for detection of Bordetella pertussis in respiratory specimens by targeting the pertactin gene. In vitro evaluation with reference strains and quality control samples showed analytical sensitivity equivalent to and specificity superior to those of PCR assays which target the IS481 element. The pertactin-based RT-PCR assay offers better discrimination between B. pertussis and other Bordetella species than previously described assays.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics*
  • Bordetella pertussis / classification
  • Bordetella pertussis / genetics
  • Bordetella pertussis / isolation & purification*
  • DNA Primers
  • Humans
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Species Specificity
  • Virulence Factors, Bordetella / genetics*
  • Whooping Cough / diagnosis*
  • Whooping Cough / microbiology


  • Bacterial Outer Membrane Proteins
  • DNA Primers
  • Virulence Factors, Bordetella
  • pertactin