Genomic analysis of protein-DNA interactions in bacteria: insights into transcription and chromosome organization

Mol Microbiol. 2007 Jul;65(1):21-6. doi: 10.1111/j.1365-2958.2007.05781.x.


Chromatin immunoprecipitation (ChIP) is a powerful method to measure protein-DNA interactions in vivo, and it can be applied on a genomic scale with microarray technology (ChIP-chip). ChIP-chip has been used extensively to map DNA-protein interactions across eukaryotic chromosomes. Here we review recent applications of ChIP-chip to the study of bacteria, which provide important and unexpected insights into transcription and chromosome organization.

Publication types

  • Review

MeSH terms

  • Bacteria / genetics
  • Bacteria / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Chromatin Immunoprecipitation / methods*
  • Chromosomes, Bacterial
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism*
  • Genome, Bacterial
  • Oligonucleotide Array Sequence Analysis / methods*
  • Transcription, Genetic


  • Bacterial Proteins
  • DNA, Bacterial