Induction of inflammatory cytokines by a keratin mutation and their repression by a small molecule in a mouse model for EBS

J Invest Dermatol. 2007 Dec;127(12):2781-9. doi: 10.1038/sj.jid.5700918. Epub 2007 Jun 21.


Epidermolysis bullosa simplex (EBS) is a skin disorder caused by mutations in keratin (K) 5 or K14 genes. It is widely regarded as a mechanobullous disease, resulting from a weakened cytoskeleton, causing extensive cytolysis. It was postulated by others that certain K14 mutations induce tumor necrosis factor-alpha (TNF-alpha) and increase apoptosis. Here, we report that in K5-/- mice and in a cell culture model of EBS, the mRNA and protein levels of TNF-alpha remain unaltered. Transcriptome analysis of K5-/- mice revealed, however, that the proinflammatory cytokines IL-6 and IL-1beta were significantly upregulated at the mRNA level in K5-/- mouse skin. These results were confirmed by TaqMan real-time PCR and ELISA assays. We hypothesize that keratin mutations contribute to EBS in a mouse model by inducing local inflammation that mediates a stress response. Following clinical reports, we applied the small molecule doxycycline to K5-/- mice. We demonstrate that doxycycline extended the survival of neonatal K5-/- mice from less than 1 to up to 8 hours. Microarray and TaqMan real-time PCR showed a downregulation of matrix metalloproteinase 13 and IL-1beta, indicating an effect of doxycycline on transcription. Our data offer a novel small molecule-based therapy approach for EBS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytokines / metabolism*
  • Disease Models, Animal
  • Doxycycline / pharmacology
  • Epidermolysis Bullosa Simplex / genetics*
  • Epidermolysis Bullosa Simplex / metabolism
  • Gene Expression Regulation*
  • Inflammation*
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Keratin-5 / genetics*
  • Keratin-5 / physiology
  • Matrix Metalloproteinase 13 / metabolism
  • Mice
  • Mutation*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic


  • Cytokines
  • Interleukin-1beta
  • Interleukin-6
  • Keratin-5
  • Matrix Metalloproteinase 13
  • Doxycycline