Resistance to Anticoccidial Drugs of Dutch Avian Eimeria Spp. Field Isolates Originating From 1996, 1999 and 2001

Avian Pathol. 2003 Aug;32(4):391-401. doi: 10.1080/0307945031000121149.


Fifteen Eimeria spp. field isolates sampled on Dutch broiler farms were subjected to an Anticoccidial Sensitivity Test (AST) in a battery cage study. Four isolates dated from 1996, another four from 1999 and the last seven isolates from 2001. The selected anticoccidial drugs were monensin, narasin, salinomycin, lasalocid, nicarbazin, diclazuril, halofuginone, maduramicin and meticlorpindol/methylbenzoquate. Maduramicin and halofuginone were not included in the ASTs of 1999 and 2001, while meticlorpindol/methylbenzoquate was not tested in 1996 and 1999. Eimeria acervulina present in each of the four 1996 field isolates showed resistance for almost all products tested except maduramicin (1/4) and salinomycin (114), which appeared to be reduced sensitive. In 1999 the same species presented a similar resistance pattern for most products, although reduced sensitivity occurred for salinomycin (1/4), and sensitivity was found for diclazuril (2/4), monensin (1/4) and narasin (1/4). In the year 2001 increased sensitivity to various products was found. Higher sensitivity was found for meticlorpindol/ methylbenzoquate (7/7) and salinomycin and narasin (both 4/7), followed by nicarbazin (3/7) and monensin (2/7). Reduced sensitivity was found for monensin (3/7), lasalocid (2/7), salinomycin and narasin (1/7). E. maxima was only found in one field isolate per year. The E. maxima from 1996 was resistant to all products except narasin (sensitive) and halofuginone (reduced sensitive). In 1999 this species was reduced sensitive to narasin and lasalocid, showing resistance for the other products. The strain originating from the 2001 isolate was reduced sensitive to most products except monensin and narasin (resistant). Full sensitivity was found for meticlorpindol/ methylbenzoquate. E. tenella was present in one isolate of 1996, two of 1999 and four isolates of 2001. The AST of 1996 showed reduced sensitivity for nicarbazin, and sensitive to narasin, maduramicin and halofuginone. All other products showed resistance. In 1999 both strains showed resistance to all products tested. For the year 2001 full sensitivity was found to meticlorpindol/methylbenzoquate. Sensitivity was also found for salinomycin (1/4), nicarbazin (2/4), diclazuril (2/4) and lasalocid (2/4), monensin (1/4) and narasin (1/4). Reduced sensitivity was found for nicarbazin (1/4), lasalocid (1/4) and narasin (1/4). The different resistance patterns of Dutch coccidiosis isolates and resistance of coccidia in general is discussed.

MeSH terms

  • Animals
  • Chickens / parasitology
  • Coccidiosis / epidemiology
  • Coccidiosis / parasitology
  • Coccidiosis / veterinary
  • Coccidiostats / pharmacology*
  • Drug Resistance*
  • Eimeria / drug effects*
  • Male
  • Netherlands / epidemiology
  • Poultry Diseases / epidemiology
  • Poultry Diseases / parasitology
  • Time Factors


  • Coccidiostats