Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappaB and p38 MAPK in HMC-1 human mast cell line

Inflamm Res. 2007 May;56(5):210-5. doi: 10.1007/s00011-007-6172-9.

Abstract

Objective and design: Mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce production of pro-inflammatory cytokines with immune regulatory properties. We investigated the effect of quercetin on the expression of pro-inflammatory cytokines in human mast cell line, HMC-1.

Methods: HMC-1 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187 (PMACI).

Results: Quercetin decreased the gene expression and production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, and IL-8 in PMACI-stimulated HMC-1 cells. Quercetin attenuated PMACI-induced activation of NF-kappaB and p38 mitogen-activated protein kinase.

Conclusion: Our study provides evidence that quercetin may suitable for the treatment of mast cell-derived allergic inflammatory diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcimycin / pharmacology
  • Carcinogens / pharmacology
  • Cell Line
  • Cytokines / metabolism*
  • Enzyme Activation / drug effects
  • Humans
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Ionophores / pharmacology
  • Mast Cells / cytology
  • Mast Cells / drug effects
  • Mast Cells / metabolism*
  • NF-kappa B / metabolism*
  • Quercetin / pharmacology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Necrosis Factor-alpha / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

  • Carcinogens
  • Cytokines
  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8
  • Ionophores
  • NF-kappa B
  • Tumor Necrosis Factor-alpha
  • Calcimycin
  • Quercetin
  • p38 Mitogen-Activated Protein Kinases
  • Tetradecanoylphorbol Acetate