Ki-67 cell proliferation in familial and in esporadic unilateral retinoblastoma: case report

Arq Bras Oftalmol. 2007 Mar-Apr;70(2):347-9. doi: 10.1590/s0004-27492007000200029.


Purpose: Ki-67 is a nuclear protein that is expressed at all phases of the cell cycle except the resting phase. This study is a clinicopathologic observational case report that aims to report on the cell proliferation rates, as measured by the Ki-67 antigen, in two enucleated retinoblastoma eyes.

Methods: One unilateral familial (mother with unilateral disease - patient 1) and one unilateral sporadic retinoblastoma (patient 2) patients were submitted to enucleation without previous treatment. The tumor cell proliferation rate was assessed by the Ki-67 antigen labeling index (stained cells / 100 cells) in five different fields of the tumor.

Results: Patient 1 was 23 months old and the tumor was exophytic with associated neovascularization of the iris; patient 2 was 6 years old and the tumor was endophytic with coarse vitreous seeds. Both enucleated eyes presented optic nerve with free surgical margins. Positive Ki-67 cell index in patient 1 varied from 75 to 90 (MD +/- SD: 79.5 +/- 6.61) and in patient 2 from 38 to 60 (MD +/- SD: 46.6 +/- 8.2).

Conclusions: The familial retinoblastoma, besides the earlier age presentation, showed 45.8% more Ki-67 positive cells than the same stage sporadic one. This proliferation rate may explain the earlier presentation age of the tumor in the inherited disease.

Publication types

  • Case Reports

MeSH terms

  • Biomarkers / analysis
  • Cell Proliferation
  • Child
  • Eye Enucleation
  • Female
  • Humans
  • Infant
  • Ki-67 Antigen / analysis*
  • Male
  • Pedigree
  • Retinal Neoplasms / pathology*
  • Retinal Neoplasms / surgery
  • Retinoblastoma / pathology*
  • Retinoblastoma / surgery
  • Retinoblastoma Protein / analysis


  • Biomarkers
  • Ki-67 Antigen
  • Retinoblastoma Protein