Abstract
Using a TaqMan gene probe targeting the 16S rDNA of acidophilic hydrogen-producing bacteria (HPB), the abundance of this HPB in the biomass was found to increase from 0.02 to 72% in a single batch treating rice slurry waste at pH 4.5 over 130 h. The corresponding abundances were 4.4% in the batch operated at pH 5.0 and 0.01-0.02% at pH 5.5-6.5. During the growth phase, the generation time for the acidophilic HPB at pH 4.5 averaged 3.5 h.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria, Anaerobic / classification
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Bacteria, Anaerobic / genetics*
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Bioreactors
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DNA Probes*
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics*
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Hydrogen / metabolism
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Phylogeny
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Sewage
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Taq Polymerase / metabolism
Substances
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DNA Probes
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DNA, Bacterial
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DNA, Ribosomal
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Sewage
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Hydrogen
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Taq Polymerase
Associated data
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GENBANK/AY862510
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GENBANK/AY862511
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GENBANK/AY862512
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GENBANK/AY862513
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GENBANK/AY862514
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GENBANK/AY862515