Analysis of protein complexes using mass spectrometry

Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54. doi: 10.1038/nrm2208.


The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Chromatography, Affinity*
  • Mass Spectrometry*
  • Multiprotein Complexes / analysis*
  • Multiprotein Complexes / isolation & purification
  • Multiprotein Complexes / metabolism
  • Proteomics*


  • Multiprotein Complexes