Susceptibilities of 82 bollworm, Helicoverpa zea (Boddie), and 44 tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae), populations to Cry2Ab2 protein were measured in diet incorporated assays at the University of Arkansas from 2002 to 2005. Resulting data were used to calculate overall (pooled data) estimates of species susceptibility for future benchmarks of resistance. Variabilities among populations also were studied by comparing regressions for individual populations and calculating mean susceptibilities for different subgroups of the colonies studied. Individual lethal concentration (LC50) estimates for nine laboratory, seven laboratory-cross, and 28 field populations of H. virescens varied up to 48-fold when adjusted for the response of the most susceptible laboratory colony studied. Mean susceptibilities of all laboratory, laboratory-cross, or field colonies varied only two-fold. When grouped by host plants, populations collected on tobacco, Nicotiana tabacum (L.), seemed to be less susceptible than those collected on other host plants. Individual LC50 values for 82 laboratory, laboratory-cross and field populations of H. zea varied up to 37-fold. Mean LC50 values of all laboratory, laboratory-cross, or field populations varied only three-fold. Susceptibilities of populations from Bollgard cotton were up to four-fold less than those from Bacillus thuringiensis corn, Zea mays L. Field populations collected during late season were generally less susceptible than those collected early in the season. Across the two species, H. zea was less sensitive to Cry2Ab2 than H. virescens. Both species seem to be less sensitive to Cry2Ab2 than to CrylAc.