Neonatal chlamydial infection induces mixed T-cell responses that drive allergic airway disease

Am J Respir Crit Care Med. 2007 Sep 15;176(6):556-64. doi: 10.1164/rccm.200607-1005OC. Epub 2007 Jun 28.


Rationale: Chlamydial lung infection has been associated with asthma in children and adults. However, how chlamydial infection influences the development of immune responses that promote asthma remains unknown.

Objectives: To determine the effect of chlamydial infection at various ages on the development of allergic airway disease (AAD).

Methods: Mouse models of chlamydial lung infection and ovalbumin-induced AAD were established in neonatal and adult BALB/c mice. Neonatal or adult mice were given a chlamydial infection and 6 weeks later were sensitized and subsequently challenged with ovalbumin. Features of AAD and inflammation were compared between uninfected or unsensitized controls.

Measurements and main results: Mild Chlamydia-induced lung disease was observed 10-15 days after infection, as evidenced by increased bacterial numbers and histopathology in the lung and a reduction in weight gain. After 6 weeks, infection and histopathology had resolved and the rate of weight gain had recovered. Neonatal but not adult infection resulted in significant decreases in interleukin-5 production from helper T cells and by the numbers of eosinophils recruited to the lung in response to ovalbumin exposure. Remarkably, the effects of early-life infection were associated with the generation of both type 1 and 2 ovalbumin-specific helper T-cell cytokine and antibody responses. Furthermore, although neonatal infection significantly attenuated eosinophilia, the generation of the mixed T-cell response exacerbated other hallmark features of asthma: mucus hypersecretion and airway hyperresponsiveness. Moreover, infection prolonged the expression of AAD and these effects were restricted to early-life infection.

Conclusions: Early-life chlamydial infection induces a mixed type 1 and 2 T-cell response to antigen, which differentially affects the development of key features of AAD in the adult.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Asthma / etiology
  • Asthma / immunology*
  • Asthma / pathology
  • Chlamydia / isolation & purification
  • Chlamydia Infections / immunology*
  • Chlamydia Infections / pathology
  • Colony Count, Microbial
  • Cytokines / immunology
  • Disease Models, Animal
  • Disease Progression
  • Female
  • Follow-Up Studies
  • Immunity, Cellular / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Pregnancy
  • Prognosis
  • T-Lymphocytes / immunology*


  • Cytokines