Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells

Pharm Res. 2008 Feb;25(2):475-80. doi: 10.1007/s11095-007-9359-4. Epub 2007 Jun 30.

Abstract

Purpose: To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.

Materials and methods: PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4.

Results: PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells.

Conclusion: The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Biological Transport
  • Carrier Proteins / physiology*
  • Cell Polarity
  • Humans
  • LLC-PK1 Cells
  • Membrane Proteins
  • Organic Anion Transporters, Sodium-Independent / physiology*
  • Phosphoproteins / physiology*
  • Placenta / metabolism
  • Sodium-Hydrogen Exchangers / physiology*

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Organic Anion Transporters, Sodium-Independent
  • PDZK1 protein, human
  • Phosphoproteins
  • SLC22A11 protein, human
  • Sodium-Hydrogen Exchangers
  • sodium-hydrogen exchanger regulatory factor