Ga-As (808 nm) laser irradiation enhances ATP production in human neuronal cells in culture

Photomed Laser Surg. 2007 Jun;25(3):180-2. doi: 10.1089/pho.2007.2064.


Objective: The aim of the present study was to investigate whether Ga-As laser irradiation can enhance adenosine triphosphate (ATP) production in normal human neural progenitor (NHNP) cells in culture.

Methods: NHNP were grown in tissue culture and were treated by Ga-As laser (808 nm, 50 mW/cm(2), 0.05 J/cm(2)), and ATP was determined at 10 min after laser application.

Results: The quantity of ATP in laser-treated cells was 7513 +/- 970 units, which was significantly higher (p < 0.05) than the non-treated cells, which comprised 3808 +/- 539 ATP units.

Conclusion: Laser application to NHNP cells significantly increases ATP production in these cells. These findings may explain the beneficial effects of low-level laser therapy (LLLT) in stroked rats. Tissue culture of NHNP cells might offer a good model to study the mechanisms associated with promotion of ATP production in the nervous system by LLLT.

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Adenosine Triphosphate / radiation effects
  • Cells, Cultured
  • Humans
  • Low-Level Light Therapy*
  • Neurons / chemistry
  • Neurons / cytology*
  • Neurons / radiation effects
  • Stem Cells / chemistry
  • Stem Cells / radiation effects*


  • Adenosine Triphosphate