Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species

Rev Iberoam Micol. 2007 Jun;24(2):148-51. doi: 10.1016/s1130-1406(07)70031-1.

Abstract

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida / classification*
  • Candida / genetics
  • Candida / growth & development
  • Candida / isolation & purification
  • Candida / metabolism
  • Candidiasis / microbiology*
  • Carbohydrate Metabolism
  • Colorimetry
  • DNA Primers
  • DNA, Fungal / analysis*
  • DNA, Fungal / genetics
  • Humans
  • Mexico
  • Mycology / methods
  • Random Amplified Polymorphic DNA Technique*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Fungal