Abstract
The major virulence determinant in clostridial myonecrosis caused by Clostridium perfringens is a phospholipase C (PLC), the alpha-toxin. Previously, mice have been protected against challenge with heterologous alpha-toxin or Clostridium perfringens spores by immunisation with the C-domain (known as Cpa(247-370) or alpha-toxoid) of the alpha-toxin. In this study, we have determined the ability of the alpha-toxoid to protect against the lethal effects of a divergent C. perfringens alpha-toxin and against the PLCs of C. absonum or C. bifermentans, species which have been isolated from cases of clostridial myonecrosis. Protection against the C. perfringens alpha-toxin variant, the C. absonum alpha-toxin or the C. bifermentans PLC was elicited by immunisation with the alpha-toxoid in vivo.
MeSH terms
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Animals
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Bacterial Toxins / immunology*
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Bacterial Vaccines / immunology
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Bacterial Vaccines / pharmacology*
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Calcium-Binding Proteins / immunology*
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Cattle
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Clostridium Infections / enzymology
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Clostridium Infections / immunology
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Clostridium Infections / microbiology
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Clostridium Infections / prevention & control*
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Clostridium bifermentans / enzymology*
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Clostridium perfringens / immunology*
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Cross Reactions
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Female
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Mice
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Mice, Inbred BALB C
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
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Type C Phospholipases / antagonists & inhibitors*
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Type C Phospholipases / immunology
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Type C Phospholipases / metabolism
Substances
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Bacterial Toxins
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Bacterial Vaccines
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Calcium-Binding Proteins
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Recombinant Proteins
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Type C Phospholipases
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alpha toxin, Clostridium perfringens