Activation of GABA(B) receptors reverses spontaneous gating deficits in juvenile DBA/2J mice

Psychopharmacology (Berl). 2007 Oct;194(3):361-9. doi: 10.1007/s00213-007-0845-5. Epub 2007 Jun 29.

Abstract

Rationale: Gamma-amino-butyric acid (GABA)(B) receptors play a key role in the pathophysiology of psychotic disorders. We previously reported that baclofen, the prototypical GABA(B) agonist, elicits antipsychotic-like effects in the rat paradigm of prepulse inhibition (PPI) of the startle, a highly validated animal model of schizophrenia.

Objectives: We studied the role of GABA(B) receptors in the spontaneous PPI deficits displayed by DBA/2J mice.

Materials and methods: We tested the effects of baclofen (1.25-5 mg/kg, intraperitoneal [i.p.]) in DBA/2J and C57BL/6J mice, in comparison to the antipsychotic drugs haloperidol (1 mg/kg, i.p.) and clozapine (5 mg/kg, i.p.). Furthermore, we investigated the expression of GABA(B) receptors in the brain of DBA/2J and C57BL/6J mice by quantitative autoradiography.

Results: Baclofen dose-dependently restored PPI deficit in DBA/2J mice, in a fashion similar to the antipsychotic clozapine (5 mg/kg, i.p.). This effect was reversed by pretreatment with the GABA(B) antagonist SCH50211 (50 mg/kg, i.p.). In contrast, baclofen did not affect PPI in C57BL/6J mice. Finally, quantitative autoradiographic analyses assessed a lower GABA(B) receptor expression in DBA/2J mice in comparison to C57BL/6J controls in the prefrontal cortex and hippocampus but not in other brain regions.

Conclusions: Our data highlight GABA(B) receptors as an important substrate for sensorimotor gating control in DBA/2J mice and encourage further investigations on the role of GABA(B) receptors in sensorimotor gating, as well as in the pathophysiology of psychotic disturbances.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antipsychotic Agents / pharmacology
  • Autoradiography
  • Baclofen / administration & dosage
  • Baclofen / pharmacology
  • Brain / metabolism
  • Brain / physiopathology*
  • Clozapine / pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • GABA Agonists / administration & dosage
  • GABA Agonists / pharmacology
  • Haloperidol / pharmacology
  • Injections, Intraperitoneal
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Neural Inhibition / drug effects
  • Neural Inhibition / physiology
  • Receptors, GABA-B / drug effects
  • Receptors, GABA-B / metabolism*
  • Reflex, Startle / drug effects
  • Reflex, Startle / physiology*
  • Schizophrenia / drug therapy
  • Schizophrenia / physiopathology*

Substances

  • Antipsychotic Agents
  • GABA Agonists
  • Receptors, GABA-B
  • Baclofen
  • Clozapine
  • Haloperidol