B cell lymphoma-associated chromosomal translocation involves candidate oncogene lyt-10, homologous to NF-kappa B p50

Cell. 1991 Dec 20;67(6):1075-87. doi: 10.1016/0092-8674(91)90285-7.


A B cell lymphoma-associated chromosomal translocation, t(10;14)(q24;q32), juxtaposes the immunoglobulin C alpha 1 locus to a novel gene, lyt-10. The normal lyt-10 cDNA codes for a 98 kd protein which displays amino-terminal homology with the rel (DNA-binding) domain of the NF-kappa B-rel family of transcription factors and carboxy-terminal homology with the NF-kappa B p50 precursor protein, including the putative proteolytic cleavage domain (poly-G) and the ankyrin-like repeat domains. The lyt-10 protein can bind to kappa B sequences in vitro, although with different specificity from NF-kappa B p50, and in vitro DNA-binding is activated by removal of the ankyrin domain. Chromosomal translocation generates an lyt-10-C alpha 1 fusion gene coding for a protein that retains the rel effector domain, lacks the ankyrin regulatory domain, and binds kappa B sequences in vitro, suggesting its constitutive activation in vivo. Analogous rearrangements of the lyt-10 gene have been found in an additional three cases of lymphoid neoplasia. The lyt-10 gene defines a new subfamily (rel/poly-G/ankyrin) of NF-kappa B-rel transcription factors with potential for oncogenic activation in human cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosomes, Human, Pair 10*
  • Chromosomes, Human, Pair 14*
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / genetics*
  • Gene Expression
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin alpha-Chains / genetics
  • Lymphoma, B-Cell / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • NF-kappa B / metabolism
  • Oligodeoxyribonucleotides / chemistry
  • Oncogenes*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Transcription Factors / genetics*
  • Translocation, Genetic


  • DNA-Binding Proteins
  • Immunoglobulin alpha-Chains
  • NF-kappa B
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Transcription Factors
  • DNA