Abstract
The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.
(c) 2007 Wiley Periodicals, Inc.
MeSH terms
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Amino Acid Sequence
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Animals
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Catalysis
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Cattle
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Cysteine / chemistry
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Disulfides / chemistry
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Hydrogen Bonding
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Kinetics
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Models, Chemical
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Molecular Sequence Data
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Protein Conformation
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Protein Folding
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Protein Structure, Secondary
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Ribonuclease, Pancreatic / analysis*
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Ribonuclease, Pancreatic / chemical synthesis*
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Ribonuclease, Pancreatic / chemistry
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Ribonuclease, Pancreatic / isolation & purification
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Water / chemistry
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X-Ray Diffraction
Substances
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Disulfides
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Water
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Ribonuclease, Pancreatic
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Cysteine